Mouse anti-human Immunoglobulin G (IgG) Monoclonal antibody

Immunoglobulin G (IgG) is a monomeric immunoglobulin, built of two heavy chains γ and two light chains. Each IgG has two antigen binding sites. It is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. IgG molecules are synthesised and secreted by plasma B cells. IgG antibodies are predominately involved in the secondary antibody response, which occurs approximately one month following antigen recognition, thus the presence of specific IgG generally corresponds to maturation of the antibody response. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in utero. Along with IgA secreted in the breast milk, residual IgG absorbed through the placenta provides the neonate with humoral immunity before its own immune system develops.
 

The antibody is a Mouse monoclonal antibody raised against IgG. It has been selected for its ability to recognize IgG in ELISA Kit and western blotting.
 

WB=1:100-500 ELISA=1:500-1000 Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
 

Store at -20°C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-8°C.
 

Shipped at 2-8°C. Upon delivery aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
 

PBS pH 7.4, 50% Glycerol, 0.02% Sodium
 

This product is for research use only, not for diagnostic or therapeutic use.