Rabbit anti-human Alpha-fetoprotein (AFP) PolyClonal antibody

AFP is a glycoprotein with a molecular weight of between 65,000 and 70,000 daltons including 4% of carbohydrate . During fetal development, AFP maintains high levels in the serum and drops to very low levels throughout the remainder of life. AFP is elevated in the malignant diseases of hepatocellular, testicular nonseminomatous origin, and occasionally of other entodermal origin. AFP may be slightly elevated or persisted in the patients with large hepatic metastases or viral hepatitis. AFP measurement is widely accepted as tumor marker and for monitoring the therapeutic effectiveness of hepatocellular cancer and nonseminomatous testicular cancer .
 

The antibody is a Rabbit polyclonal antibody raised against AFP. It has been selected for its ability to recognize AFP in ELISA Kit and western blotting.
 

WB=1:100-500 ELISA=1:500-1000 Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
 

Store at -20°C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-8°C.
 

Shipped at 2-8°C. Upon delivery aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
 

0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
 

This product is for research use only, not for diagnostic or therapeutic use.