Product Details
生产种属 |
Human |
来源 |
Prokaryotic expression |
宿主 |
E. coli |
同义词 |
GFP; EGFP |
浓度 |
1 mg/mL |
形态 |
Lyophilized or Liquid |
纯度 |
> 95% as determined by SDS-PAGE |
序列 |
C5MKY7 |
标签 |
N-terminal His-tag and Trx-ta |
纯化方式 |
Affinity purification |
分子量 |
26 kDa |
应用 |
Western blotting,ELISA;SDS-PAGE;Antigen; |
产品概述
The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range.Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm.
使用方法
Reconstitute in PBS or others.
稳定性
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
存储
Store it under sterile conditions at -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -80℃.
存储缓冲液
Lyophilized from 50mM Tris-HCL,0.5mM EDTA,50mM NaCl,pH 7.4
注意事项
This product is for research use only, not for diagnostic or therapeutic use.