Product Details
生产种属 |
Human |
来源 |
Prokaryotic expression |
宿主 |
E. coli |
同义词 |
Apo-E; APOE; ApoE |
浓度 |
1 mg/mL |
形态 |
Lyophilized or Liquid |
纯度 |
> 90% as determined by SDS-PAGE |
序列 |
Lys19~Gln181 (P02649) |
标签 |
N-terminal His-tag |
纯化方式 |
Affinity purification |
分子量 |
20 kDa |
应用 |
Western blotting,ELISA;SDS-PAGE;Antigen; |
产品概述
Apolipoprotein E (ApoE) is a class of apolipoprotein found in the chylomicron and Intermediate-density lipoprotein (IDLs) that is essential for the normal catabolism of triglyceride-rich lipoprotein constituents.[1] In peripheral tissues, ApoE is primarily produced by the liver and macrophages, and mediates cholesterol metabolism in an isoform-dependent manner. In the central nervous system, ApoE is mainly produced by astrocytes, and transports cholesterol to neurons via ApoE receptors, which are members of the low density lipoprotein receptor gene family.
使用方法
Reconstitute in PBS or others.
稳定性
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
存储
Store it under sterile conditions at -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -80℃.
存储缓冲液
Lyophilized from 20mM PB, 300mMNaCl,pH7.7
注意事项
This product is for research use only, not for diagnostic or therapeutic use.