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Danofloxacin ELISA Kit

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  • Cat.No.: AE81070SF

  • Reactivity: N/A

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48T 96T
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Product Details

种属 N/A
UniProt号 N/A
缩写 Danofloxacin
别名 N/A
线性范围 0.1-8.1 ppb
灵敏度 0.1 ppb
样本类型 Tissue, aquatic, honey, egg, etc.
检测方法 Competitive ELISA
分析方法 Quantitive
反应时间 1-3h
样本体积 10-200 μL
检测波长 450 nm

原理

This assay is based on the competitive enzyme immunoassay for the detection of Danofloxacin in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Danofloxacin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Danofloxacin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Danofloxacin in it. This value is compared to the standard curve and the Danofloxacin concentration is subsequently obtained.
 

产品概述


 

组份


Reagents

Quantity

Reagents

Quantity

Assay plate (96 Wells)

1

Instruction manual

1

Standard (lyophilized)

2

Sample Diluent

1 x 20 mL

Biotin-Conjugate (concentrate 100 x)

1 x 120 μL

Biotin-Conjugate Diluent

1 x 12 mL

Streptavidin-HRP  (concentrate 100 x)

1 x 120 μL

Streptavidin-HRP Diluent   

1 x 12 mL

Wash Buffer (concentrate 25 x)

1 x 20 mL

Substrate Solution

1 x 10 mL

Stop Solution

1 x 6 mL

Adhesive Films

4

 

特异性


 

回收率


Sample Type

Number

Recovery range (%)

Average(%)

Serum

10

90-101

96

EDTA plasma

10

89-97

93

Heparin plasma

10

91-99

95

 

精密度

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100 Intra-Assay: CV<8% Inter-Assay: CV<12%
 

线性


Sample Type

1：2

1：4

1：8

1：16

Serum

78-89%

81-99%

92-103%

95-105%

EDTA plasma

91-101%

90-98%

93-101%

91-98%

Heparin plasma

92-103%

93-102%

92-99%

91-101%

 

稳定性

The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
 

样本收集及储存

Store at 2-8°C. Please refer to Instruction Manual.
 

注意事项

Store at 2-8°C. Please refer to Instruction Manual.
 

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